We collected soil samples on three days that represented the early
(June 11), peak (July 9), and late (August 16) growing season in 2018.
We collected integrated, duplicated soil samples from the organic soil
layer (Oe and Oa; -5-0 cm) and across every 10 cm down to 1 m in two
soil pits, but only analyzed one sample for isotopic composition: one
pit sampled adjacent (< 5 m) to reference
plots (n = 1 soil sample for each depth; n = 11 samples on each date;
n = 33 samples total throughout the growing season) and one pit
adjacent (< 5 m) to treatment plots (warmed and warmed +
FTC; n = 1 soil sample for each depth; n = 11 samples on
each date; n = 33 samples total throughout the growing season). Pits
were 2 m wide and 1 m deep and samples were collected from the edge of
the pit facing the plots. We gently scraped away soil from the 2 cm of
the soil pit exposed to air (area of soil facing the opening of the
soil pit) prior to sample collection throughout the 0-1 m depth soil
pit in case evaporation occurred from the side of the pit that was
exposed to air. Soil samples were placed directly into scintillation
vials and stored in a cooler with ice. Once in the laboratory, all
samples were frozen until analysis.
Water from soil was extracted using a cryogenic vacuum distillation
system at the Boston University Stable Isotope Lab (Boston, MA, USA).
The extraction time was approximately 60 minutes for soil samples. All
soil water samples were analyzed for stable isotopic composition using
a Liquid Isotope Water Analyzer (LIWA) cavity ringdown spectrometer
(Model DLT-100; Los Gatos Research, Mountain View, CA, USA) at
Plymouth State University (Plymouth, NH, USA). For each date, the
stable isotope ratios of hydrogen and oxygen in each water sample were
determined and expressed in standard delta notation
(δ2H and δ18O,
respectively, ‰). We corrected for narrow and broadband spectral
interference due to the presence of organic compounds using the
LGR-SCI software (Version 1.0.0.69; Schultz et al., 2011).
Schultz, N.M., Griffis, T.J., Lee, X., Baker, J.M., 2011.
Identification and correction of spectral contamination in 2H/ 1H and
18O/ 16O measured in leaf, stem, and soil water. Rapid Commun. Mass
Spectrom. 25, 3360–3368. https://doi.org/10.1002/rcm.5236