Data Package Metadata View Summary

Multiple Element Limitation in Northeast Hardwood Ecosystems (MELNHE) - raw images for the analysis of stomatal density and length 2021-2022

General Information

Data Package:

Local Identifier:

knb-lter-hbr.321.1

Title:

Multiple Element Limitation in Northeast Hardwood Ecosystems (MELNHE) - raw images for the analysis of stomatal density and length 2021-2022

Alternate Identifier:

DOI PLACE HOLDER

Abstract:

Stomatal density and length were measured on leaves of sugar maple (Acer sacharrum Marsh.) and yellow birch (Betula alleghaniensis Britton.) trees in New Hampshire at the Bartlett Experimental Forest, Hubbard Brook Experimental Forest, and Jeffers Brook as part of the Multiple Elementation Limitation in Northern Hardwood Ecosystems (MELNHE) study. Leaves were collected in late July and early August in 2021 and 2022 from the tops of dominant and codominant trees using a shotgun. These measurements were made on 3 leaves from each tree.

These data correspond with other foliar trait data collected from the same trees in 2021 and 2022. That EDI package is as follows:

Hong, S.D., K.E. Gonzales, C.R. See, and R.D. Yanai. 2021. MELNHE: Foliar Chemistry 2008-2016 in Bartlett, Hubbard Brook, and Jeffers Brook (12 stands) ver 1. Environmental Data Initiative. https://doi.org/10.6073/pasta/b23deb8e1ccf1c1413382bf911c6be19

This data package contains the raw images underlying the data reported in a separate data package on stomatal density and length.

These data were gathered as part of the Hubbard Brook Ecosystem Study (HBES). The HBES is a collaborative effort at the Hubbard Brook Experimental Forest, which is operated and maintained by the USDA Forest Service, Northern Research Station.

Publication Date:

2023-10-03

For more information:
Visit:	DOI PLACE HOLDER

Time Period

Begin:

2021-07-01

End:

2022-12-31

People and Organizations
Contact:	Hubbard Brook Ecosystem Study [ email ]
Creator:	Zukswert, Jenna M (SUNY ESF)
Creator:	Weimer, Sydney (SUNY ESF)
Creator:	McGarry, Alannah (SUNY ESF)
Creator:	Fessenden, Alicia (SUNY ESF)
Creator:	Yanai, Ruth D (SUNY-ESF)
Creator:	Carter, Ashley (SUNY ESF)

Data Entities
Data Table Name:	MELNHE_stomatal_images_2021-22_manifest
Description:	manifest file for sugar maple and yellow birch stomata images. file indicates the zip file containing each image
Other Name:	MELNHE_Stomatal_Density_Yellow_Birch_20x
Description:	zip file of yellow birch stomata images from 2021 pilot study with 20x magnification

Detailed Metadata

Data Entities

Data Table


Data:	https://pasta-s.lternet.edu/package/data/eml/knb-lter-hbr/321/1/f2dc5098c4502b6e9e24c5249ab897d9
Name:	MELNHE_stomatal_images_2021-22_manifest
Description:	manifest file for sugar maple and yellow birch stomata images. file indicates the zip file containing each image
Number of Records:	2710
Number of Columns:	16

Table Structure

Object Name:

MELNHE_stomatal_images_2021-22_manifest.csv

Size:

415293 byte

Authentication:

c0b3ed70518fdb0222288bbb6163c83b Calculated By MD5

Text Format:

Number of Header Lines:

Record Delimiter:

\r\n

Orientation:

column

Simple Delimited:

Field Delimiter:	,
Quote Character:	"

Table Column Descriptions

File_Name

File_Size

File_Type

MD5_Checksum

Date_Created

Plot

Species

Tree_Number

Tag_ID

Leaf_Number

Image_Number

Magnification

Comments

ZIP_folder

Column Name:

File_Name

File_Size

File_Type

MD5_Checksum

Date_Created

Stand

Plot

Treatment

Species

Tree_Number

Tag_ID

Leaf_Number

Image_Number

Magnification

Comments

ZIP_folder

Definition:

Image file name

Image file size in bytes

file format type

MD5 checksum

file creation date

Tree stand name

Plots 1-4 were assigned a N, P, NP, or Control Treatment. Plot 5 is always Ca.

Nutrient addition (CONTROL, N, P, NP, or Ca)

species

tree number

Tree tag number at the time of sampling

leaf number

image number

Magnification

comments

zip folder containing the image

Storage Type:

string

dateTime

string

Measurement Type:

nominal

dateTime

nominal

Measurement Values Domain:

Definition

text

Definition

text

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	bmp
Definition	bmp file format
Source

Code Definition

Code	jpg
Definition	jpg file format
Source

Code Definition

Code	tif
Definition	tif file format
Source

Code Definition

Code	tiff
Definition	tiff file format
Source

Code Definition

Code	xml
Definition	xml file format
Source

Definition

text

Format	MM/DD/YY hh:mm
Precision

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	C1
Definition	Bartlett Experiemental Forest young stand clearcut in 1990
Source

Code Definition

Code	C2
Definition	Bartlett Experiemental Forest young stand clearcut in 19988
Source

Code Definition

Code	C3
Definition	Bartlett Experiemental Forest young stand
Source

Code Definition

Code	C4
Definition	Bartlett Experiemental Forest middle aged stand clearcut in 1978
Source

Code Definition

Code	C5
Definition	Bartlett Experiemental Forest middle aged stand
Source

Code Definition

Code	C6
Definition	Bartlett Experiemental Forest middle aged stand clearcut in 1975
Source

Code Definition

Code	C7
Definition	Bartlett Experiemental Forest mature aged stand
Source

Code Definition

Code	C8
Definition	Bartlett Experiemental Forest mature aged stand clearcut in 1883
Source

Code Definition

Code	C9
Definition	Bartlett Experiemental Forest mature aged stand clearcut around 1890
Source

Code Definition

Code	HBM
Definition	Hubbard Brook Experimental Forest middle aged stand clearcut in 1970
Source

Code Definition

Code	HBO
Definition	Hubbard Brook Experimental Forest mature aged stand clearcut around 1900
Source

Code Definition

Code	JBM
Definition	Jeffers Brook middle aged forest stand clearcut around 1975
Source

Code Definition

Code	JBO
Definition	Jeffers Brook mature aged forest stand clearcut around 1900
Source

Code Definition

Code	HBCa
Definition	Hubbard Brook MELNHE calcium treatment plot
Source

Definition

text

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	C
Definition	No treatment
Source

Code Definition

Code	N
Definition	Nitrogen addition (30 kg N/ha/yr as NH4NO3)
Source

Code Definition

Code	P
Definition	Phosphorus addition (10 kg P/ha/yr as NaH2PO4)
Source

Code Definition

Code	NP
Definition	Nitrogen and phosphorus addition (30 kg N/ha/yr as NH4NO3 and 10 kg P/ha/yr as NaH2PO4)
Source

Code Definition

Code	Ca
Definition	Calcium addition (1150 kg Ca/ha in the form of CaSiO3 in 2011)
Source

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	SM
Definition	sugar maple
Source

Code Definition

Code	YB
Definition	yellow birch
Source

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	1
Definition	tree 1
Source

Code Definition

Code	2
Definition	tree 2
Source

Code Definition

Code	3
Definition	tree 3
Source

Code Definition

Code	4
Definition	tree 4
Source

Definition

text

Definition

text

Definition

text

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	100
Definition	magnification 100
Source

Code Definition

Code	200
Definition	magnification 200
Source

Code Definition

Code	400
Definition	magnification 400
Source

Definition

text

Definition

text

Missing Value Code:

Accuracy Report:

Accuracy Assessment:

Coverage:

Methods:

Non-Categorized Data Resource

Name:

MELNHE_Stomatal_Density_Yellow_Birch_20x

Entity Type:

zip file

Description:

zip file of yellow birch stomata images from 2021 pilot study with 20x magnification

Physical Structure Description:

Object Name:

MELNHE_Stomatal_Density_Yellow_Birch_20x.zip

Size:

103115082 byte

Authentication:

a51d143c27a7b4c3cec7196b5f22f119 Calculated By MD5

Externally Defined Format:

Format Name:

ZIP

Data:

https://pasta-s.lternet.edu/package/data/eml/knb-lter-hbr/321/1/4ebf06fe86a055a213f56317922b972c

Data Package Usage Rights

This information is released under the Creative Commons license - Attribution - CC BY (https://creativecommons.org/licenses/by/4.0/). The consumer of these data ("Data User" herein) is required to cite it appropriately in any publication that results from its use. The Data User should realize that these data may be actively used by others for ongoing research and that coordination may be necessary to prevent duplicate publication. The Data User is urged to contact the authors of these data if any questions about methodology or results occur. Where appropriate, the Data User is encouraged to consider collaboration or co-authorship with the authors. The Data User should realize that misinterpretation of data may occur if used out of context of the original study. While substantial efforts are made to ensure the accuracy of data and associated documentation, complete accuracy of data sets cannot be guaranteed. All data are made available "as is." The Data User should be aware, however, that data are updated periodically and it is the responsibility of the Data User to check for new versions of the data. The data authors and the repository where these data were obtained shall not be liable for damages resulting from any use or misinterpretation of the data. Thank you.

Keywords

By Thesaurus:
LTER Controlled Vocabulary	ecosystems, forests, foliage, disturbance, fertilization, nutrients, density
HBES Thesaurus	stomata, stomatal density, stomatal length, MELNHE, HBR, LTER, Hubbard Brook Experimental Forest, Bartlett Experimental Forest, Jeffers Brook, White Mountain National Forest

Methods and Protocols

These methods, instrumentation and/or protocols apply to all data in this dataset:

Methods and protocols used in the collection of this data package

Description:

Sunlit foliage was sampled from two locations in canopies of dominant and codominant sugar maple and yellow birch trees (>10 cm in diameter at breast height) using a shotgun in most of the plots in the Multiple Element Limitation in Northern Hardwood Ecosystems (MELNHE). More information on this project design can be found in the following file:

Yanai, R.D., M. Fisk, and T.J. Fahey. 2022. Multiple Element Limitation in Northeast Hardwood Ecosystems (MELNHE): Project description, plot characteristics and design ver 1. Environmental Data Initiative. https://doi.org/10.6073/pasta/bcfc628d26e78b3dff648c34a33da1a3

Foliage was sampled at Hubbard Brook and Jeffers Brook in 2021 and at Bartlett in 2022; foliage from 85 sugar maple trees and 137 yellow birch trees were sampled in total. Most trees were sampled from the measurement area, though occasionally trees from the buffer were sampled if there were not enough suitable trees there. Three leaves from each tree were selected for stomatal density and length measurements. These leaves were air-dried and pressed in a plant press for at least 72 hours.

Stomata were visualized using clear nail polish impressions. Clear nail polish was applied in three approximately 1 x 1-cm squares on the leaf: one near the base of the leaf, one in the middle of the leaf, and one at the top of the leaf, along but avoiding the midvein. Impressions were mounted on clear slides using transparent tape and viewed using light microscopy at 400x magnification. One photograph was taken of each impression. Care was taken to photograph an area on the impression in which as many stomata were as clear as possible and veins were minimally present, if at all. Photographed stomata were counted in each image using ImageJ software and converted to units of stomata per square millimeter by dividing the count by 0.066 square millimeters.

Length of three stomata were measured in each image for 15 yellow birch trees in JBM and for 35 sugar maple trees across HBM, HBO, and JBM (405 stomata for yellow birch, 945 stomata for sugar maple). Length was measured in micrometers.

An initial pilot study was conducted on sugar maple trees in stand HBM using 200x magnification (i.e., a 20x objective lens), and a second pilot study was done measuring stomata in yellow birch at 100x vs. 400x (10x vs. 40x objective lenses). These density and length data are included as “other entities” in this data package.

People and Organizations

Publishers:

Organization:

Environmental Data Initiative

Email Address:

info@edirepository.org

Web Address:

https://edirepository.org

Id:

https://ror.org/0330j0z60

Creators:

Individual:

Jenna M Zukswert

Organization:

SUNY ESF

Email Address:

jmzukswe@syr.edu

Id:

https://orcid.org/0000-0003-2246-0406

Individual:

Sydney Weimer

Organization:

SUNY ESF

Email Address:

sydneyweimer16@gmail.com

Individual:

Alannah McGarry

Organization:

SUNY ESF

Email Address:

amcgarry@syr.edu

Individual:

Alicia Fessenden

Organization:

SUNY ESF

Email Address:

amfessen@syr.edu

Individual:

Ruth D Yanai

Organization:

SUNY-ESF

Address:

SUNY College of Environmental Science and Forestry,

210 Marshall Hall,

Syracuse, NY 13210

Email Address:

rdyanai@syr.edu

Id:

https://orcid.org/0000-0001-6987-2489

Id:

https://ror.org/00qv0tw17

Individual:

Ashley Carter

Organization:

SUNY ESF

Email Address:

alcarter182@gmail.com

Contacts:

Organization:

Hubbard Brook Ecosystem Study

Email Address:

hbr-im@lternet.edu

Id:

https://ror.org/00mkh7345

Temporal, Geographic and Taxonomic Coverage

Temporal, Geographic and/or Taxonomic information that applies to all data in this dataset:

Time Period

Begin:

2021-07-01

End:

2022-12-31

Geographic Region:

Description:

Hubbard Brook Experimental Forest

Bounding Coordinates:

Northern:	43.959	Southern:	43.914
Western:	-71.8062	Eastern:	-71.7022

Sampling Site:

Description:

Bartlett Experimental Forest

Site Coordinates:

Longitude (degree):

-71.2973

Latitude (degree):

44.0556

Geographic Region:

Description:

Jeffers Brook MELNHE Plots

Bounding Coordinates:

Northern:	44.038	Southern:	44.034
Western:	-71.891	Eastern:	-71.888

Project

Parent Project Information:

Title:

Multiple Element Limitation in Northeast Hardwood Ecosystems (MELNHE)

Personnel:

Individual:

Ruth D Yanai

Organization:

SUNY-ESF

Address:

SUNY College of Environmental Science and Forestry,

210 Marshall Hall,

Syracuse, NY 13210

Email Address:

rdyanai@syr.edu

Id:

https://orcid.org/0000-0001-6987-2489

Id:

https://ror.org/00qv0tw17

Additional Award Information:

Funder:	National Science Foundation
Number:	0949324
Title:	Collaborative Research: Nutrient co-limitation in young and mature northern hardwood forests

Additional Award Information:

Funder:	USDA National Institute of Food and Agriculture
Number:	2019-67019-29464
Title:	Soil Nutrient Limitation of Northern Hardwood Forest Productivity: Mechanisms Contributing to N and P co-Limitations (2019)

Additional Award Information:

Funder:

National Science Foundation

Funder ID:

https://ror.org/021nxhr62

Number:

1637685

Title:

LTER: Long Term Ecological Research at the Hubbard Brook Experimental Forest (2016)

URL:

https://www.nsf.gov/awardsearch/showAward?AWD_ID=1637685

Additional Award Information:

Funder:

National Science Foundation

Funder ID:

https://ror.org/021nxhr62

Number:

1633026

Title:

Long-Term Ecological Research at the Hubbard Brook Experimental Forest (2016b)

URL:

https://www.nsf.gov/awardsearch/showAward?AWD_ID=1633026

Other Metadata

Additional Metadata

additionalMetadata
        |___text '\n    '
        |___element 'metadata'
        |     |___text '\n      '
        |     |___element 'emlEditor'
        |     |        \___attribute 'app' = 'ezEML'
        |     |        \___attribute 'release' = '2023.08.30'
        |     |___text '\n    '
        |___text '\n  '

Copyright 2024 Environmental Data Initiative. This material is based upon work supported by the National Science Foundation under grants #2223103 and #2223104. Any opinions, findings, conclusions, or recommendations expressed in the material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation. Please contact us with questions, comments, or for technical assistance regarding this web site or the Environmental Data Initiative. Please read our privacy policy to know what information we collect about you and to understand your privacy rights.

EDI is a collaboration between the University of New Mexico and the University of Wisconsin – Madison, Center for Limnology:

Data Package Metadata View Summary

Multiple Element Limitation in Northeast Hardwood Ecosystems (MELNHE) - raw images for the analysis of stomatal density and length 2021-2022

Data Entities

Data Table

Non-Categorized Data Resource

Data Package Usage Rights

Keywords

Methods and Protocols

These methods, instrumentation and/or protocols apply to all data in this dataset:

People and Organizations

Temporal, Geographic and Taxonomic Coverage

Project

Parent Project Information:

Additional Metadata

Recently Added

Recently Updated

Data Package Metadata View Summary

Multiple Element Limitation in Northeast Hardwood Ecosystems (MELNHE) - raw images for the analysis of stomatal density and length 2021-2022

+/- Data Entities

Data Table

Non-Categorized Data Resource

+/- Data Package Usage Rights

+/- Keywords

+/- Methods and Protocols

These methods, instrumentation and/or protocols apply to all data in this dataset:

+/- People and Organizations

+/- Temporal, Geographic and Taxonomic Coverage

+/- Project

Parent Project Information:

+/- Additional Metadata

Data Entities

Data Package Usage Rights

Keywords

Methods and Protocols

People and Organizations

Temporal, Geographic and Taxonomic Coverage

Project

Additional Metadata