Overview and study location
This biodiversity study was initiated in 1995 and measurements
were conducted from 1997-2002 as part of the Jornada Basin LTER
program. The objective of this study was to distinguish the
differential effects of plant community biomass, plant community
functional groups, and biodiversity within functional groups to a
number of plant community responses. To make these distinctions,
treatments were established by the selective removal of plant
species or of all species of a functional group within a plot.
Given the long lifespans and difficulty of establishment of desert
perennials, establishment of desired communities from seed or
transplant was impractical. Instead, we established our stands of
varying plant diversity by making appropriate removals of species
or species groups from replicate plots of a diverse Chihuahuan
scrub site. Treatments were established in summer/fall 1995.
Plants were removed by simple cutting at the soil surface (no soil
disturbance or herbicide). For most species, this was effective;
recruits of perennial grasses were removed from the appropriate
plots twice per year, and regrowing shrubs were clipped back as
needed. After 2 years only one perennial grass and two shrubs
required substantial effort for plot maintenance.
The experimental area is a 250 m x 250 m area located on the NMSU
Chihuahuan Desert Rangeland Research Center, immediately northeast
of the intersection of the Summerford powerline road and the road
running along the southern boundary of the Jornada Experimental
Range and the CDRRC. The area was gridded into 25 m x 25 m plots;
because of some existing environmental gradients (the area slopes
to the east, and mesquite abundance varies from north to south) 6
blocks were established and treatments were randomly assigned to
plots within blocks. Plot boundaries are marked with rebar in the
corners and simple steel wire stretched along boundaries; plots
are identified by number and treatment on aluminum tags and on
plastic rebar caps in the southwest corner.
Treatments and sampling design
There are eight treatments: a control (C, no removals); four
functional group removal treatments (PG, perennial grass removed;
S, shrubs removed; SSh, subshrubs removed; Succ, succulents
removed), and three treatments where richness within a functional
group was manipulated. The latter include the simplified treatment
(Simp, where only the single most abundant species of each growth
form remains, while all other species of those growth forms are
removed), the reduced‐Larrea treatment (rL, where the dominant of
each growth form is removed, and minority components remain), and
a second form of the reduced treatment (rP, where Prosopis rather
than Larrea is removed as the shrub dominant). To summarize:
Growth form removals
Shrub Removal (S) = all shrubs removed
(Larrea, Prosopis, Ephedra, etc.)
Subshrub Removal (Ss) = all subshrubs removed
(Zinnia, Xanthocephalum, etc.)
Perennial Grass Removal (PG) = all perennial
grasses removed (Muhlenbergia, Dasychloa, Setaria, etc.)
Succulent Removal (Succ) = all leaf and stem
succulents removed (Yucca, Opuntia, etc.)
Modifications of species richness within growth forms
Simplified (Simp) = Only a single species
(the dominant) of each growth form remaining, all subordinate
species removed; plot contains only LATR, ZIAC, MUPO, and YUBA as
woody or grass perennials.
Reduced minus Larrea (rL) = Dominant of each
growth form removed, only subordinates remain; Larrea removed as
dominant shrub.
Reduced minus Prosopis (rP) = Dominant of
each growth form removed, only subordinates remain; Prosopis
removed as dominant shrub
Control (C) = All species of all growth forms
remain.
Treatments were established in summer/fall 1995 by randomly
assigning all 8 treatments to plots within each of 6 blocks in the
experimental area (blocking to allow for spatial heterogeneity in
environment and vegetation). There are therefore 6 replicates of
each of the 8 treatments in 48 total plots. In removal treatments
plants were removed by cutting at the soil surface (no soil
disturbance or herbicide application). All vegetation removed
during treatment application (Fall 1995) was field weighed and
recorded, then converted to dry weights for later use as covariate
or measure of disturbance.
Plant removal and dry mass estimation
For any species to be removed from a plot, each individual rooted
in the plot (or that portion of an individual entering the plot
from outside) was cut at ground level, minimizing soil disturbance
where possible. Aboveground portions were weighed in the field
(fresh weight) with a dairy scale and entered in field data
sheets; conversions of data from English units to g were done in
the lab. Plant material for each species from the first 10 plots
was brought back to the lab for oven drying (45 degrees C) to
constant mass. The ratio of dry mass to fresh mass (dry mass/fresh
mass) was calculated and used to convert all other fresh weight
measurements to dry mass in grams. When a species was encountered
in a later plot, for which there had been no collection and
calculation of dry/fresh ratio, we applied the ratio from a
species of similar growth form and phenology.
Dead biomass of shrubs and subshrubs removed from a plot was
collected and weighed (assumed to be dry so no oven-drying). Dead
material that was part of a live plant was not separated from the
plant and was included in the live mass weight. Dead material was
identified as that lying on the ground or a rooted plant that was
completely dead. Dry mass of each species was summed for each
plot; then species’ masses were summed by growth form for each
plot.
This data set consists of the total dry mass (converted from
fresh) by functional group removed from each plot, plus the
combined mass of dead, live, and live + dead biomass (total)
removed from the plots.
Additional Files
An additional metadata file - bdremove.dsd.txt - from the earlier
Jornada information management system is included and may contain
useful metadata.
REFERENCES
Buonopane, Michelle, Laura Foster Huenneke, and Marta Remmenga.
"Community response to removals of plant functional groups
and species from a Chihuahuan Desert shrubland." Oikos 110.1
(2005): 67-80.