Experimental design
This study was conducted in a Bouteloua eripoda grassland in
Pasture 13 of the Jornada Experimental Range in Dona Ana County,
New Mexico, USA. In 2006, 36 2.5m x 2.5m plots were established in
each of three blocks on a level upland with flat slopes protected
from livestock grazing since 1996. The experiment was implemented
as a randomized complete block design with 2 factors:
precipitation manipulation (3 levels) and nitrogen fertilization
(2 levels).
The 3 levels of precipitation manipulation were 1) 80% reduced
precipitation, 2) ambient precipitation, and 3) 80% increased
precipitation. Reduction of 80% precipitation was achieved with
rainout shelters with transparent acrylic troughs that acted like
shingles. An irrigation system was used to implement the water
addition treatments.
The two levels of nitrogen fertilization consisted of 1) liquid
Ammonium nitrate fertilizer applied twice each growing season in
mid- and late July from 2006 to 2008 (treatment), and 2)
application of the same amount of water, equivalent to a 2mm rain
event (controls). The ammonium nitrate fertilizer consisted of 10
grams of ammonium nitrate dissolved in water per meter per year.
Treatments began in 2007.
Treatment combinations were randomly assigned to plots within each
block. Six replicates were used for each treatment combination
yielding total 36 plots per block.
Data collection and biomass calculation
Plant cover was sampled with a line intercept method in fall 2006
before any treatments began, then again in fall 2007 and 2008
after treatments had been applied. Within each plot, green
interception by species was recorded for all plants present in the
plot on 3 250cm parallel lines evenly spaced from the east border.
Plant cover was later used to calculate plot biomass and then ANPP
following procedures in Flombaum and Sala 2007, and Sala and
Austin 2000.
Related data and publications
An earlier dataset containing 2006-2009 data from these plots is
available on EDI (package ID: knb-lter-jrn.210278002), and soil
moisture data from this study can be found in EDI data package
knb-lter-jrn.210349002. Note that from 2006-2009 there were two
additional precipitation treatments that are not included in the
present dataset (+50% and -50%).
For further information and results, see:
Throop, H., L. G. Reichmann, O. Sala, and S. Archer. (2012),
Response of dominant grass and shrub species to water
manipulation: an ecophysical basis for shrub invasion in a
Chihuahuan desert grassland. Oecologia 169: 373-383.
https://doi.org/10.1007/s00442-011-2217-4
Reichmann, L. G., O. E. Sala, and D. P. C. Peters. (2013), Water
controls on nitrogen transformations and stocks in an arid
ecosystem. Ecosphere 4(1):11. https://doi.org/10.1890/ES12-00263.1
Reichmann, L.G., Sala, O.E. and Peters, D.P.C. (2013),
Precipitation legacies in desert grassland primary production
occur through previous‐year tiller density. Ecology, 94: 435-443.
https://doi.org/10.1890/12-1237.1