Methods in brief
During winter 1987, eight harvester ant (Pogonomyrmex rugosus)
nests at site 1, five harvester ant nests at site 2 and seven ant
nests at site 3 were randomly selected and systematically sampled.
At each nest, a 0.16 square meter quadrat frame was randomly
placed at the ant nest edge, a second 0.16 square meter quadrat
was also placed on the ant nest edge opposite to the first
quadrat. Two additional quadrat frames were placed 5 meters
distance from the two at the edge of the ant nests. These quadrats
represent the soil unmodified by the activity of the ants and
served as a reference.
On 24 June 1987, soil samples were collected from five ant nests
for each of the three sites for analysis of total nitrogen,
(ammonium, nitrite, nitrate), inorganic phosphorus, and
exchangeable cations (K+, Na+, Ca2+ and Mg2+). The two quadrats of
the ant nest edges were randomly sampled once with a 400-cm3 core
(20 cm depth) and the soil samples were mixed together. The same
procedures were used for the two quadrats of adjacent reference
sites.
All chemical analyses were determined on the < 2-mm soil
fraction. Total nitrogen was measured by microKjeldahl digestion
(Bremmer and Mulvaney, 1982) and analyzed twice. Ammonium (NH4-N),
nitrite (NO3-N), and nitrate (NO3-N) were extracted with 100 ml of
2 N KCl on 10-g soil samples and were measured as described by
Keeney and Nelson (1982). Inorganic phosphorus (P) was extracted
with 100 ml of 0.5 M NaHCO3 on approximately 10-g soil samples and
was measured on an autoanalyzer. Exchangeable cations (K+, Na+,
Ca2+ and Mg2+) were extracted with 1 N ammonium acetate (pH 7.0)
and were analyzed with an atomic absorption spectrophotometer. On
24 June 1987, below ground biomass was measured at only five ant
nests from each of the three sites. A 400 cm3 soil core sample was
taken from each quadrat (edges and reference). Roots were cleaned
with a 2-mm sieve, oven-dried for 72 hours at 60 C and carefully
weighed.
Variables in the dataset include chemical analyses for soil
samples collected from five ant nests for each of the three sites
for total nitrogen, (ammonium, nitrate), inorganic phosphorus, and
exchangeable cations (K+, Na+, Ca2+ and Mg2+). Also included is
below ground biomass from five ant nests for each of the three
sites.
Additional Files
An additional metadata file - ansnutr.dsd.txt - from the earlier
Jornada information management system is included and may contain
useful metadata.