One hundred and ten fluff grass plants were transplanted into pots,
divided into 5 replicates of each of 4 treatments: 1) control, 2)
NEMACUR (to exclude nematodes), 3) chlordane (to exclude mites) and 4)
NEMACUR + Chlordane. Replicates were assigned to one of 5 sampling
times (0,2,4,8,12 weeks), and transferred to a green house. Pots were
watered weekly (6mm/week) to maintain them at field capacity. For each
sampling time, rhizosphere soil was analyzed for NO3(-)- N, NH4(+)-N,
Inorganic-N, microbial-N, % H20, % Organic matter, roots biomass, and
nematode density.
Microarthropods were extracted in modified Tullgren funnels into
water, then counted and identified to species. After microarthropod
extraction the soil was sieved through a 2-mm screen mesh and roots
were collected. On subsamples for nematode extraction, roots were
carefully separated by hand, then nematodes were extracted by a
modified sugar flotation technique (Freckman et al. 1977). Remaining
soil was sieved through a 2-mm screen mesh.
Inorganic N (NH4 -N and NO3 +NO2 -N) was extracted by placing 10 g
sub-sample of each soil sample in polyethylene bottles containing 100
ml of 2.0 Molar KCL + PMA ( to prevent growth by bacteria and fungi)
(Keeney and Nelson 1982). Sub-samples were shaken 30 times and
filtered after setting overnight NH4 -N was measured in the extracts
by an automated salicylate procedure (Wall and Gehrke 1975, Nelson
1983). NO3 + NO2 -N was measured using an automated cadmium reduction
procedure (Henriksen and Selmer-Olsen 1970). Automated measurements
were made on a Scientific Instruments Continuous Flow Analyzer.