Twenty 6 x 6 m plots with a 3-meter buffer zone were established on
the Jornada Basin CDRCC in 1986. Five plots each were randomly
assigned to one of four treatments: (1) chlordane amendment (100ml Al
(Active Ingredients) per 10,000 ml) to exclude microarthropods, (2)
water (irrigation 6 mm per week), (3) water (6 mm per week) plus
chlordane amendment (100 ml Al per 10,000 ml) and (4) control, no
treatment.
An ion exchange resin bag technique (Binkley 1984, Lajtha 1988) was
used to determine N availability in the rhizosphere of Erioneuron
pulchellum. A 50 cm2 area of undyed nylon stocking material was sewn
into a bag containing 10 g (wet weight) of either Dowex 1-X8 anion
exchange resin or Dowex 50 W- X8 cation exchange resin, both 20-50
mesh. Anion resins were placed in three successive rinses of 0.5 M
NaHCCO3, converting resins to the bicarbonate form. Cation resin bags,
already in the H+ form, were rinsed three successive times with dilute
HC1. All bags were rinsed with deionized water and spun dry in an
open-basket hand centrifuge before being taken to the field. Ten bags
of anion and two bags of cation exchange resin were placed in each
plot (total 20 plots), directly in the rhizosphere of a plant (12
Erioneuron pulchellum plants per plot) at approximately 10 cm depth.
Bags were replaced every 12 weeks for 9 months. Bags were rinsed
thoroughly in deionized water and spun dry upon collection from the
field. Anion and cation bags were desorbed in 2.0 M KC1 + PMA (to
avoid bacterial and fungal growth). Samples were shaken 30 times, let
set overnight, and filtered. The solution was analyzed for NH4-N using
an automated salicylate procedure (Wall and Gehrke, 1975; Nelson 1983)
and NO3 + NO2 -N using an automated cadmium reduction procedure
(Henriksen and Selmer- Olsen 1970). Standards containing anion or
cation exchange resin bags were extracted the same as field exchange
resin bags.