Twenty 6 x 6 m plots with a 3-meter buffer zone were established at
the Chihuahuan Desert Rangeland Research Center (CDRRC)in the Jornada
Basin in 1986. Five plots each were randomly assigned to one of four
treatments: (1) chlordane amendment (100ml AI (Active Ingredients) per
10,000 ml) to exclude microarthropods, (2) water (irrigation 6 mm per
week), (3) water (6 mm per week) plus chlordane amendment (100 ml AI
per 10,000 ml) and (4) control, no treatment.
An ion exchange resin bag technique (Binkley 1984, Lajtha 1988) was
used to determine N availability in the rhizosphere of fluffgrass
(
Dasyochloa pulchella
). A 50 cm2 area of undyed
nylon stocking material was sewn into a bag containing 10 g (wet
weight) of either Dowex 1-X8 anion exchange resin or Dowex 50 W- X8
cation exchange resin, both 20-50 mesh. Anion resins were placed in
three successive rinses of 0.5 M NaHCCO3, converting resins to the
bicarbonate form. Cation resin bags, already in the H+ form, were
rinsed three successive times with dilute HC1. All bags were rinsed
with deionized water and spun dry in an open-basket hand centrifuge
before being taken to the field. Eight bags of anion and two bags of
cation exchange resin were placed in each plot (total 20 plots),
directly in the rhizosphere of a plant (12 Dasyochloa pulchella plants
per plot) at approximately 10 cm depth. Bags were replaced every 12
weeks for 9 months. Bags were rinsed thoroughly in deionized water and
spun dry upon collection from the field. Anion and cation bags were
desorbed in 2.0 M KC1 + PMA (to avoid bacterial and fungal growth).
Samples were shaken 30 times, let set overnight, and filtered. The
solution was analyzed for NH4-N using an automated salicylate
procedure (Wall and Gehrke, 1975; Nelson 1983) and NO3 + NO2 -N using
an automated cadmium reduction procedure (Henriksen and Selmer-Olsen
1970). Standards containing anion or cation exchange resin bags were
extracted the same as field exchange resin bags.