Experimental design
The experimental design for the Cross-Scale Interactions study
(CSIs) consists of 15 - 1 ha blocks spatially distributed across
the study area selected to have similar total percentage
vegetation cover within a block, but to vary in the proportion of
perennial grass and mesquite cover among blocks. At each block
location, four 15m x 25m plots were identified with similar
percentage grass and shrub cover with a focal patch in the center.
Plots were arrayed perpendicular lengthwise to the dominant
southwest to northeast wind direction, and were >15 m apart to
minimize between-plot treatment effects.
Each plot within a block was randomly assigned to one of four
connectivity treatments: (1) plant scale where all mesquite plants
within and surrounding the focal patch were killed in place to
modify competitive interactions between woody plants and recovery
of perennial grasses and other herbaceous plants with no direct
effects on horizontal transport by wind and water, (2) patch scale
where Connectivity Modifiers (ConMods, Okin et al. 2015) were
located in bare soil interspaces between plants in the focal patch
to reduce gap size and to modify transport of water, soil,
nutrients, litter, and herbaceous seeds, (3) both patch- and
plant-scale manipulations were conducted in each focal patch, and
(4) no manipulations [controls]. Block and plot selection were
completed in June 2012 followed by the characterization of initial
vegetation cover in all plots in June 2013 when treatments were
initiated.
Assignment of treatments to experimental plots (by block) is
described in the attached file "csis_treatment_key.csv".
Overhead photo collection
In each plot (4 per block), repeat overhead photos of ten randomly
selected microplots were photographed annually. Photos were taken
from directly above the microplot (overhead, or downward-looking
orientation) from a height of approximately 85cm above ground
level using a 12.1 megapixel digital camera, resulting in image
dimensions of 4,000 x 3,000 pixels. In ConMod-only and ConMod +
Herbicide treatments, photos were centered over the center post of
the ConMod structure, and in Control and Herbicide-only plots
photos were centered over a marker nail affixed to the soil
surface. Annual photos were taken in winter (Jan-Feb) from
2013-2016 to try to better distinguish perennial grass from annual
plant biomass. In 2017 the method changed and photos were taken
near peak biomass during the growing season (July-Sept) in order
to better capture and identify living biomass. Images were
archived for later analysis of plant growth and litter
accumulation within the microplots.
Before cover analysis, photos were standardized by cropping, and
in some cases by rotating, for use in USDA SamplePoint software
(Booth et al. 2006, Peters et al. 2020). Photos in this dataset
are the ones used in these later analyses in the study and are
archived by year in zip archives. A directory to the photos is
provided in the file "jrn413004_photo_archive_dir.csv".
References
Okin, Gregory S., Mariano Moreno-de las Heras, Patricia M. Saco,
Heather L. Throop, Enrique R. Vivoni, Anthony J. Parsons, John
Wainwright, and Debra P. C. Peters. 2015. "Connectivity in
Dryland Landscapes: Shifting Concepts of Spatial
Interactions." Frontiers in Ecology and the Environment 13
(1): 20–27. https://doi.org/10.1890/140163.
Booth, D. Terrance, Samuel E. Cox, and Robert D. Berryman.
"Point sampling digital imagery with 'SamplePoint'."
Environmental Monitoring and Assessment 123 (2006): 97-108.
Peters, Debra P. C., Gregory S. Okin, Jeffrey E. Herrick, Heather
M. Savoy, John P. Anderson, Stacey L. P. Scroggs, and Junzhe
Zhang. 2020. "Modifying Connectivity to Promote State Change
Reversal: The Importance of Geomorphic Context and Plant–Soil
Feedbacks." Ecology 101 (9): e03069.
https://doi.org/10.1002/ecy.3069.