These methods, instrumentation and/or protocols apply to all data in this dataset:Methods and protocols used in the collection of this data package |
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Description: | Field methods:
Leaf packs of Tabonucco leaves were air dried at room temperature for 5-7 days, with leaves rotated at least twice a day to promote equal drying. Leaves were then grouped into packs of 5 ± 0.6 grams and clipped together at the petiole using a binder clip. Each leaf pack was attached to a wire frame with plastic zip ties and secured by steel rebars hammered into the stream substrate. Leaf pack were collected on days, 3, 7, 14, 22, 29, 36, 43, and 50. Leaf packs were collected by placing a small net downstream of the pack as it was removed from the frame to avoid loss of invertebrates or leaf material. The pack and any invertebrates collected in the net were then placed in a Ziplock bag and transported to the lab on ice. Retrieved leaf packs were rinsed over a 250 μm sieve, and any insects dislodged were placed in ethanol vials and labeled for future identification. On two dates leaf packs were not collected and are indicated as n/a for all organisms. |
| Description: | Lab Methods:
All organisms were identified to the lowest taxonomic level in the lab (in most cases to the family level) using the keys Gutiérrez-Fonseca, P.E., Alonso-Rodríguez, A.M., & Ramirez, A. (2018). Macroinvertebrados acuáticos de Puerto Rico como bioindicadores de la calidad ambiental. https://doi.org/10.5281/zenodo.1425834 and Ramírez, A.M and Gutiérrez-Fonseca, P.E. Freshwater Macroinvertebrates of the Caribbean (In prep.). |
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