Sampling
Samples of Sargassum pacificum and Turbinaria ornata. are collected from
fringing, backreef, and reef crest habitats at each LTER site (LTER 1 through LTER
6) around Moorea. Fringing communities were defined as reefs adjacent to the shore
and within ca. 50 m of the land. The backreef is located within the lagoon and may
be synonymous with lagoon habitats in other studies. The reef crest is the narrow
section of partially exposed (during low tide) reef that divides the backreef and
outer reef. Individuals (n=10 years 2005-2014, 2020-2021; n=5 years 2017-2019, 2022) of each
species are collected from each habitat at each site. Samples are returned to the
lab and approximately 1 g (wet mass) of tissue is removed (5 cm from a branch apex)
from each individual, rinsed in fresh water (FW), and dried to constant weight at 80
degrees C. Dried samples are submitted for CHN analysis using standard techniques.
Ratios of C:N are calculated using their weight percent.
Description: Organic Elemental Analyzer - CHN:
The model CEC 440HA features: high-temperature combustion analysis and
simultaneous determination C, H, and N as weight percents. This instrument
determines "organic carbon, hydrogen, and nitrogen by means of high-temperature
(1000C) combustion (Dumas method) of microgram to milligram size samples in an
oxygen-enriched helium atmosphere. The combustion products (CO2, NOX (reduced to
N2), and H2O), after chemical removal of potentially interfering gasses, are
separated by means of sequential, selective chemical traps, and measured with three
pairs of high-precision thermal conductivity detectors. Samples, which have been
precisely weighed into small aluminum capsules, placed in protective nickel sleeves,
and loaded into the autosampler wheel, are introduced into the combustion furnace by
means of a mechanically operated quartz ladle. When combustion is complete, the
ladle is withdrawn, and the sample and capsule residue in the nickel sleeve is
deposited in a waste chamber (also sealed under helium during the run), thus
avoiding buildup of sample residue in the combustion tube. Analysis results are
reported in weight percent of the element." (from
http://www.msi.ucsb.edu/services/analytical-lab/instruments/organic-elemental-analyzer-chn)