Data Package Metadata View Summary

Lake Mendota, Wisconsin, USA, Zebra Mussel Veliger Water Column Density 2016-2019

General Information

Data Package:
Local Identifier:	knb-lter-ntl.392.1
Title:	Lake Mendota, Wisconsin, USA, Zebra Mussel Veliger Water Column Density 2016-2019
Alternate Identifier:	DOI PLACE HOLDER
Abstract:	We sampled veliger (larval stage) zebra mussels (Dreissena polymorpha) from 2016-2019. Zebra mussels are invasive in Lake Mendota and were first detected in November 2015. Samples were taken at three different sites on Lake Mendota from June to August in 2016, and from June to November in 2018-2019, using a 0.5 m diameter, 64 micrometer mesh size plankton net for an 8 m depth tow. This dataset complements adult zebra mussel, zoobenthos, and phytobenthos data collected during the same time period, for which data is also archived with EDI.
Publication Date:	2020-07-28

Time Period

Begin:

2016-06-01

End:

2019-11-30

People and Organizations
Contact:	Vander Zanden, Jake (Center for Limnology, University of Wisconsin-Madison) [ email ]
Creator:	Spear, Michael J (Center for Limnology, University of Wisconsin-Madison)
Creator:	Wakker, Petra (Center for Limnology, University of Wisconsin-Madison)
Creator:	Vander Zanden, Jake (Center for Limnology, University of Wisconsin-Madison)
Associate:	Shannon, Thomas (Center for Limnology, University of Wisconsin-Madison, Field Crew)
Associate:	Norris, Ella (Center for Limnology, University of Wisconsin-Madison, Field Crew)
Associate:	McKittrick, Dane (Center for Limnology, University of Wisconsin-Madison, Field Crew)
Associate:	Zamora, Adeline (Center for Limnology, University of Wisconsin-Madison, Field Crew)
Associate:	Schiereck, Samantha (Center for Limnology, University of Wisconsin-Madison, Field Crew)

Data Entities
Data Table Name:	veligers_EDI.csv
Description:	Zebra mussel density at each sampling

Detailed Metadata

Data Entities

Data Table


Data:	https://pasta-s.lternet.edu/package/data/eml/knb-lter-ntl/392/1/c20adca8cecac33770b0d659da7267ff
Name:	veligers_EDI.csv
Description:	Zebra mussel density at each sampling
Number of Records:	262
Number of Columns:	10

Table Structure

Object Name:

veligers_EDI.csv

Size:

12300 bytes

Authentication:

7ae6883e30afe10f44ac615b3c68efe1 Calculated By MD5

Text Format:

Number of Header Lines:

Record Delimiter:

\r\n

Orientation:

column

Simple Delimited:

Field Delimiter:	,
Quote Character:	"

Table Column Descriptions

Column Name:

date

year

transect

replicate

depth

initial_scan

vol_sample

mean_count_ml

vol_watercolumn

density_m3

Definition:

Date sample was collected

Year sample was collected

Transect (developed for adult zebra mussel sampling) along which the 10 m depth site was visited for veliger collection

Samples were collected in triplicate at most sites on each date

Depth at which the plankton net was lowered before towing it to the surface, i.e., the length of the plankton tow

Result of the initial cross-polarized microscopy scan at low-magnification to determine presence/absence of veligers

Volume of the condensed sample from which 1 mL subsamples were drawn. Used together with mean_count_ml, and vol_watercolumn, to calculate density_m3

Mean number of veligers found in each of the five 1 mL subsamples used for enumeration

Volume of the water column sampled by the plankton net. Essentially the volume of the cylinder created by the 0.5 diameter plankton net towed to the surface from a depth of 8 m.

Estimated density of veligers in the water column

Storage Type:

date

float

string

float

string

float

Measurement Type:

dateTime

ratio

nominal

ratio

nominal

ratio

Measurement Values Domain:

Format	YYYY-MM-DD
Precision

Unit	nominalYear
Type	natural
Min	2016
Max	2019

Definition

Transect (developed for adult zebra mussel sampling) along which the 10 m depth site was visited for veliger collection

Unit	dimensionless
Type	natural
Min	1
Max	3

Unit	meter
Type	natural
Min	8
Max	8

Allowed Values and Definitions

Enumerated Domain

Code Definition

Code	1
Definition	veligers present
Source

Code Definition

Code	0
Definition	veligers absent
Source

Unit	milliliter
Type	natural
Min	15
Max	200

Unit	individualsPerMilliliter
Type	real
Min	0
Max	147

Unit	cubicMeter
Type	real
Min	1.57
Max	1.57

Unit	individualsPerCubicMeter
Type	real
Min	0
Max	7677.707006

Missing Value Code:

Code	NA
Expl	not available

Code	NA
Expl	not available

Code	NA
Expl	not available

Code	NA
Expl	not available

Code	NA
Expl	not available

Code	NA
Expl	not available

Code	NA
Expl	not available

Accuracy Report:

Accuracy Assessment:

Coverage:

Methods:

Data Package Usage Rights

This information is released under the Creative Commons license - Attribution - CC BY (https://creativecommons.org/licenses/by/4.0/). The consumer of these data (\"Data User\" herein) is required to cite it appropriately in any publication that results from its use. The Data User should realize that these data may be actively used by others for ongoing research and that coordination may be necessary to prevent duplicate publication. The Data User is urged to contact the authors of these data if any questions about methodology or results occur. Where appropriate, the Data User is encouraged to consider collaboration or co-authorship with the authors. The Data User should realize that misinterpretation of data may occur if used out of context of the original study. While substantial efforts are made to ensure the accuracy of data and associated documentation, complete accuracy of data sets cannot be guaranteed. All data are made available \"as is.\" The Data User should be aware, however, that data are updated periodically and it is the responsibility of the Data User to check for new versions of the data. The data authors and the repository where these data were obtained shall not be liable for damages resulting from any use or misinterpretation of the data. Thank you.

Keywords

By Thesaurus:
(No thesaurus)	Zebra mussel, veliger, NTL-LTER, Lake Mendota, North Temperate Lakes LTER
LTER Controlled Vocabulary	zooplankton, invasive species

Methods and Protocols

These methods, instrumentation and/or protocols apply to all data in this dataset:

Methods and protocols used in the collection of this data package

Description:

We sampled larval zebra mussels (veligers) using a 64 microm mesh, 0.5 m diameter plankton net and stored them in 80% ethanol in 200 mL containers at 25degree C for 0-12 weeks until processing. At each site we performed triplicate 8 m depth plankton tows by pulling a net from 2 m above the lake bottom at the 10 m depth sites of transects A-C developed for adult zebra mussel collection. We collected samples approximately every 14 days from June to August in 2016, and June to November in 2017-2019. During fall sampling, poor weather conditions occasionally limited the number of sites or replicates collected. We also sampled veligers biweekly in 2019 but reduced sampling to one replicate per site and only sampled at one site after September. Because veligers are small and difficult to see, enumeration was time consuming.

We used a preliminary presence/absence scan to eliminate samples without veligers before investing time in enumeration. For the initial scan, we removed 10 mL subsamples from homogenized zooplankton samples and scanned for veligers under cross-polarized microscopy at low magnification (10X-40X). We scanned five 10 mL subsamples before declaring veligers absent in a sample. For samples with confirmed veliger presence, we returned scanned zooplankton material to the sample bottle, concentrated sample volume to 50-150 mL, re-homogenized the sample, and enumerated five 1 mL subsamples at 400X magnification under both cross-polarized and unpolarized light in Sedgewick-Rafter counting cells. We used the average of these five replicates (x ̅_1mL) the volume (mL) of the concentrated sample (VS), and the volume (m-3) of lake water sampled by the plankton net (VL; pi * (0.5 m diameter net / 2)2 * 8 m net tow) to calculate veliger abundance as a water column density (# m-3) for each replicate at each site according to the following equation.

Density=(x_1mL*V_S)/V_L

People and Organizations

Creators:

Individual:

Michael J Spear

Organization:

Center for Limnology, University of Wisconsin-Madison

Email Address:

mspear2@wisc.edu

Id:

https://orcid.org/0000-0002-1365-3583

Individual:

Petra Wakker

Organization:

Center for Limnology, University of Wisconsin-Madison

Email Address:

wakker@wisc.edu

Individual:

Jake Vander Zanden

Organization:

Center for Limnology, University of Wisconsin-Madison

Email Address:

mjvanderzand@wisc.edu

Id:

https://orcid.org/0000-0002-8122-1416

Contacts:

Individual:

Jake Vander Zanden

Organization:

Center for Limnology, University of Wisconsin-Madison

Email Address:

mjvanderzand@wisc.edu

Id:

https://orcid.org/0000-0002-8122-1416

Associated Parties:

Individual:

Thomas Shannon

Organization:

Center for Limnology, University of Wisconsin-Madison

Email Address:

tshan018@fiu.edu

Role:

Field Crew

Individual:

Ella Norris

Organization:

Center for Limnology, University of Wisconsin-Madison

Email Address:

enorris2@wisc.edu

Role:

Field Crew

Individual:

Dane McKittrick

Organization:

Center for Limnology, University of Wisconsin-Madison

Email Address:

dmckittrick@wisc.edu

Role:

Field Crew

Individual:

Adeline Zamora

Organization:

Center for Limnology, University of Wisconsin-Madison

Email Address:

azamora2@wisc.edu

Role:

Field Crew

Individual:

Samantha Schiereck

Organization:

Center for Limnology, University of Wisconsin-Madison

Email Address:

sschiereck@wisc.edu

Role:

Field Crew

Temporal, Geographic and Taxonomic Coverage

Temporal, Geographic and/or Taxonomic information that applies to all data in this dataset:

Time Period

Begin:

2016-06-01

End:

2019-11-30

Geographic Region:

Description:

Lake Mendota, Madison, WI, USA

Bounding Coordinates:

Northern:	43.148805	Southern:	43.075119
Western:	-89.486789	Eastern:	-89.36654

Taxonomic Range:

Classification:

Rank Name:

kingdom

Rank Value:

Animalia

Classification:

Rank Name:

subkingdom

Rank Value:

Bilateria

Classification:

Rank Name:

infrakingdom

Rank Value:

Protostomia

Classification:

Rank Name:

superphylum

Rank Value:

Lophozoa

Classification:

Rank Name:

phylum

Rank Value:

Mollusca

Classification:

Rank Name:

class

Rank Value:

Bivalvia

Classification:

Rank Name:

subclass

Rank Value:

Heterodonta

Classification:

Rank Name:

order

Rank Value:

Veneroida

Classification:

Rank Name:

superfamily

Rank Value:

Dreissenoidea

Classification:

Rank Name:

family

Rank Value:

Dreissenidae

Classification:

Rank Name:

genus

Rank Value:

Dreissena

Classification:

Rank Name:	species
Rank Value:	Dreissena polymorpha

Project

Parent Project Information:

Title:

The zebra mussel invasion of Lake Mendota: New insights into invasive species eruptions and impact

Personnel:

Individual:

Jake Vander Zanden

Email Address:

mjvanderzand@wisc.edu

Id:

https://orcid.org/0000-0002-8122-1416

Role:

Principal Investigator

Funding:

Wisconsin Department of Natural Resources AIRD11318

Related Project:

Title:

North Temperate Lakes – Long Term Ecological Research program

Personnel:

Individual:

Emily Stanley

Email Address:

ehstanley@wisc.edu

Id:

https://orcid.org/0000-0003-4922-8121

Role:

Principal Investigator

Funding:

National Science Foundation DEB?1440297

Maintenance

Maintenance:

Description:	completed
Frequency:

Other Metadata

Additional Metadata

additionalMetadata
        |___text '\n    '
        |___element 'metadata'
        |     |___text '\n      '
        |     |___element 'unitList'
        |     |     |___text '\n        '
        |     |     |___element 'unit'
        |     |     |     |  \___attribute 'id' = 'individualsPerMilliliter'
        |     |     |     |  \___attribute 'multiplierToSI' = ''
        |     |     |     |  \___attribute 'name' = 'individualsPerMilliliter'
        |     |     |     |  \___attribute 'parentSI' = ''
        |     |     |     |  \___attribute 'unitType' = ''
        |     |     |     |___text '\n          '
        |     |     |     |___element 'description'
        |     |     |     |___text '\n        '
        |     |     |___text '\n        '
        |     |     |___element 'unit'
        |     |     |     |  \___attribute 'id' = 'individualsPerCubicMeter'
        |     |     |     |  \___attribute 'multiplierToSI' = ''
        |     |     |     |  \___attribute 'name' = 'individualsPerCubicMeter'
        |     |     |     |  \___attribute 'parentSI' = ''
        |     |     |     |  \___attribute 'unitType' = ''
        |     |     |     |___text '\n          '
        |     |     |     |___element 'description'
        |     |     |     |___text '\n        '
        |     |     |___text '\n      '
        |     |___text '\n    '
        |___text '\n  '

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EDI is a collaboration between the University of New Mexico and the University of Wisconsin – Madison, Center for Limnology:

Data Package Metadata View Summary

Lake Mendota, Wisconsin, USA, Zebra Mussel Veliger Water Column Density 2016-2019

Data Entities

Data Table

Data Package Usage Rights

Keywords

Methods and Protocols

These methods, instrumentation and/or protocols apply to all data in this dataset:

People and Organizations

Temporal, Geographic and Taxonomic Coverage

Project

Parent Project Information:

Maintenance

Additional Metadata

Recently Added

Recently Updated

Data Package Metadata View Summary

Lake Mendota, Wisconsin, USA, Zebra Mussel Veliger Water Column Density 2016-2019

+/- Data Entities

Data Table

+/- Data Package Usage Rights

+/- Keywords

+/- Methods and Protocols

These methods, instrumentation and/or protocols apply to all data in this dataset:

+/- People and Organizations

+/- Temporal, Geographic and Taxonomic Coverage

+/- Project

Parent Project Information:

+/- Maintenance

+/- Additional Metadata

Data Entities

Data Package Usage Rights

Keywords

Methods and Protocols

People and Organizations

Temporal, Geographic and Taxonomic Coverage

Project

Maintenance

Additional Metadata