The primary aim of the data product is to quantify seasonal and spatial variation in sediment phosphorus fluxes in a temperate reservoir and evaluate mechanisms responsible for instances of elevated sediment phosphorus release. We studied Green Valley Lake, a hypereutrophic reservoir in southwest Iowa, USA, from 2019 to 2020. We measured sediment phosphorus flux rates and potential explanatory variables at three sites along the longitudinal gradient of the reservoir over six sampling events during winter and summer stratification as well as mixing events in the spring, summer, and fall. Ex situ sediment core incubations were used to measure sediment P release rates under ambient temperature and dissolved oxygen conditions. Explanatory variables measured included sediment phosphorus chemistry, sediment physical characteristics, epilimnetic and hypolimnetic nutrient concentrations, and thermal stratification patterns. These data will be used to identify mechanisms driving hot spots and hot moments of sediment phosphorus release, which will contribute to our understanding of how areas of lakebed and times of the year can disproportionately influence whole-lake water chemistry.